Title PhD Position in infection biology

Job title:

Title PhD Position in infection biology

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Job description

Offer DescriptionProject title: Systems biology analysis of multi-scale innate immune defences against bacterial pathogensFunded by the National Agency for Academic ExchangeWe are seeking enthusiastic and highly motivated scientists to join a newly established laboratory of prof. Pawel Paszek. Successful candidate will work in a team using interdisciplinary approaches to study single cell immune responses to bacterial infections.The main aim of the project is to quantitatively understand mechanisms involved in the muti-scale coordination of innate immune responses against bacterial pathogens at the single cell level. We will study direct interactions between innate immune macrophages and the important food-borne bacteria Listeria monocytogenes that causes significant levels of human morbidity and mortality. Our data demonstrate that interactions between L. monocytogenes and macrophages, a key event controlling the overall infection critically rely on a small fraction of ‘successful’ pathogens, which can effectively replicate and spread within the infected host cell population.Our main objectives are:

• Elucidate via time-lapse live-cell imaging how pathogen threats are encoded in dynamical responses of NF-kB/STAT/IRF systems upon L. monocytogenes infection.
• Understand the population-level host strategies that underpin successful immune response to infection. We will investigate how paracrine signalling in the infected and bystander population and temperature adaptation coordinate the overall population level inflammatory responses.
• Determine whether the host and pathogen variability are co-regulated as a fundamental immune mechanism. We will use image genomics (smFISH) and immunostaining to measure single-cell effector responses in host macrophages as well as monitor activation of the major PrfA regulon in L. monocytogenes at the single bacterium resolution.

Develop predictive mathematical models of innate immune response to L. monocytogenes using genetic and chemical perturbations to validate modelling and manipulate infection outcomes.Where to apply E-mailppaszek@ippt.pan.plRequirementsResearch Field Biological sciences Education Level Master Degree or equivalentSkills/QualificationsWe seek excellent candidates having experience in one of the following areas: * Immunology, microbiology or molecular cell biology, with basic knowledge in experimental techniques: mammalian and/or bacterial cell culture, live and fixed-cell confocal microscopy, RT or digital PCR, Western blot, cell sorting, molecular cloning and CRISPR. Experience in other methods planned for the project and described below is desirable.

• Mathematical and numerical system biology, ordinary and partial differential equations, stochastic processes, programming, image analysis.

Background in both areas will be considered beneficial.Specific RequirementsThe successful candidate will be trained in the following methodologies (subject to his/her background and interests):

• Live-cell confocal imaging using L. monocytogenes encoding fluorescent proteins and reporters of Prfa virulence, human and mouse cells (primary as well as immortalized macrophage lines) expressing fluorescently tagged transcription factors and cytokines.
• Immunostaining. Simultaneous measurement of regulatory proteins involved in regulation of immune responses in single cells.
• Gene expression, protein analysis and cytokine production in infected cell populations through the use of RT-PCR, digital PCR, Western Blotting, ELISA, generation of reporter lines using molecular cloning and CRISPR, and other relevant techniques.

Mathematical modeling employing Markov processes to describe activation of the immune response in heterogeneous cell populations, cellular communication, density and temperature effect, different outcomes of infection (eradication, persistence, replication), regulation of bacterial virulence.Languages ENGLISH Level ExcellentResearch Field Biological sciences Years of Research Experience 1 – 4Additional InformationBenefits

• 6000 PLN/month brutto brutto for 36 months (depending on the position and type of employment contract).
• Opportunity to join a doctoral school such as TIB PAN

, which will provide additional financial support. * The project provides unique opportunities for interdisciplinary research in the field of infection biology.

• Working on high-class research equipment with experienced staff as a member of Laboratory of Modelling in Biology and Medicine (

).IPPT PAN is a top interdisciplinary research institute in Poland located in a beautiful and very international city, WarsawEligibility criteriaCandidates must hold a MSc degree in a relevant field and have excellent track record for their career stage.Selection processPlease provide the following documentation with your application:1. Copy of MSc diploma in relevant field. Bachelor diplomas may be also included for evaluation.2. CV containing information about:

• Publications,
• Conference presentations,
• Prizes and stipends,
• Other information that can help in evaluation, like experience, achievements, and scientific standing of the candidate.
• Contact details for two references,
• Please include in your CV the following clause: “I agree to the processing of personal data contained in my job offer for the needs necessary to carry out the recruitment process conducted by IPPT PAN with headquarters in Warsaw, ul. A. Pawińskiego 5B, according to art. 13 para. 1 and 2 of Regulation (EU) 2016/679 of the Parliament and of the Council of 27 April 2016 on the protection of individuals with regard to the processing of personal data and the free movement of such data and the repeal of Directive 95/46 / EC (RODO).

3. Motivation letter (1 page).The documents should be sent till 31st of August 2024 to Project PI, Prof. Pawel Paszek ( ) with a copy to Department Secretary Ms Agnieszka Ponarska ( ). Suitable candidates can be contacted and invited for an interview every fortnight in order to fill the position as soon as possible.Website for additional job detailsWork Location(s)Number of offers available 1 Company/Institute Institute of Fundamental Technological Research Polish Academy of Sciences Country Poland State/Province Mazovia City Warsaw Postal Code 02-106 Street A.Pawińskiego 5BContact State/ProvinceMazovia CityWarsaw WebsiteStreetA.Pawińskiego 5B Postal Code02-106 E-Mailamilcz@ippt.pan.plSTATUS: EXPIREDShare this page

Expected salary

Location

Warszawa, mazowieckie

Job date

Thu, 25 Jul 2024 06:02:28 GMT

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