Job title:
Research specialist position (role in the project: Technician)
Company:
Job description
Research specialist position (role in the project: Technician)Recruitment for the project “RNA and Cell Biology Platform for Research and Innovation in Medicine (RACE-PRIME)” (grant agreement no. FENG.02.01-IP.05-T003/23) implemented within the framework of the International Research Agendas funded by the European Funds for Smart Economy programme, for the position of Research specialist to carry out research work in the cell biology area under the direction of Prof. Marta Miączyńska, head of the Laboratory of Cell Biology at the International Institute of Molecular and Cell Biology in Warsaw ( ), in collaboration with Dr Aleksandra Kołodziejczyk (Laboratory of Cellular Genomics).The aim of RACE-PRIME is to establish IIMCB as a world-class Center of Excellence in RNA and Cell Biology. The goal of RACE-PRIME is to translate basic research findings, e.g., for developing new therapies, diagnostic methods, discovering new therapeutic targets, or identifying molecules with desired properties to be developed into drugs.The impact of the gut microbiota on human health is undeniable. The liver is particularly interesting in the context of the microbiota because of its exposure to bacterially derived products. Together with nutrients from digested food, microbiota-derived molecules and metabolites, reach the liver via portal circulation. This makes the liver uniquely exposed to microbiota, without having direct contact with live bacteria.In the gut, we have hundreds of different bacterial strains and each of them produces many different products that can be beneficial or detrimental to the host. Our understanding of which bacteria exert such positive or negative effects is limited. Hence, there is a need for a system that will allow testing the effects of bacteria in a high throughput way. Studying interactions between microbiota and liver is usually done in animal models, which greatly limits the number of samples. Hence, we plan to establish an in vitro model with the key characteristics of the gut-liver axis and then use it to test if and how bacterially derived metabolites act on the liver.Job description: Our overarching aim is to gain a comprehensive understanding of the molecular and cellular mechanisms underlying the gut-liver axis in health and disease with the goal of improving clinical outcomes. The goals of this study are: (1) to establish a transwell-based cell culture system to mimic the gut-liver axis, (2) to identify bacterial strains producing metabolites that pass through the epithelial barrier and impact the liver, and (3) to characterise mechanisms of this interaction.In this project, you will use – advanced multicomponent mammalian cell cultures, genomics and microbiological techniques such as anaerobic bacteria culturing. There will be an opportunity to work with mice as animal models of gastrointestinal diseases.Requirements: in biological sciences or related field. * knowledge of molecular and cell biology
- hands-on experience in laboratory work
- prior experience in following techniques will be an advantage (but not a prerequisite)
- Sequencing libraries generation (RNAseq, single-cell genomics, metagenomics)
- Cell culture (cell lines, organoids, CRISPR-based cell line modification techniques)
- Microbiology (culturing bacterial isolates, phenotyping, working anaerobically)
- Working with mice (handling, injections, oral gavage, dissection, perfusion)
- Independence, commitment, initiative, teamwork skills.
- Proficiency in spoken and written English.
Selection criteria: * Technical competencies, experience and alignment of specialized knowledge with the position.
- Scientific achievements.
- Soft skills – proficiency in English, motivation letter, communication skills, teamwork ability, alignment of individual development plans with project goals.
Candidates will be selected through an open competition, in accordance with the principles set out in the European Charter for Researchers and the Code of Conduct for the Recruitment of Researchers, which are part of the HR Excellence in Research strategy.We offer: * Full-time employment contract, initially for a period of 6 months with the possibility of extension.
- Competitive salary in the amount of 6000 PLN gross/month.
- Attractive benefits package including subsidization for MultiSport card and medical insurance.
- Full technical, administrative, and organizational support in Polish and English from professional staff.
- Opportunity to participate in courses, conferences, scientific training, and substantive support from colleagues.
- Opportunity to do a PhD in an extramural form.
How to apply: * Please send your applications to the email address:
- In the email subject line, please include the following information: “CELL 5 Technician MAB” followed by your first and last name.
- Applications should be submitted in English. The application should include a CV,
list of achievements, a motivation letter describing key scientific achievements, and contact details (email and phone number) of at least two potential referees, including your direct supervisor. * Please include the following statement in your CV: “I hereby give consent for my personal data included in the application documents to be processed by the International Institute of Molecular and Cell Biology in Warsaw, ul. Ks. Trojdena 4, 02-109 Warsaw, for the purpose of carrying out the recruitment process.” Full information is available at
.The competition may be extended until a candidate meeting all requirements is found.
Expected salary
Location
Warszawa, mazowieckie
Job date
Wed, 17 Jul 2024 06:41:36 GMT
To help us track our recruitment effort, please indicate in your email/cover letter where (vacanciesineu.com) you saw this job posting.
