Research logistician/Engineer position (with PhD) in flow cytometry

Job title:

Research logistician/Engineer position (with PhD) in flow cytometry

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Job description

Offer DescriptionPositionFull-time (100%) research logistician/engineer (fixed-term) in flow cytometry for the flow cytometry core facility of UNamur (Belgium)Work environmentThe Morph-Im (Morphology & Imaging) core facility ( ) is one of the nine technological platforms of Université de Namur and is dedicated to offer the state-of-the art in imaging technologies, both for life science and surface material applications. The platform gathers a wide variety of state-of-the-art instruments and expertise, such as electron and optical microscopy, atomic force microscopy, scanning tunneling microscopy, histology and flow cytometry. Morph-Im includes now 3 flow cytometers (2 analysers and 1 cell sorter).Your missions

• Management of the users, computers and reagents

• Daily and annual maintenance

• Contacts with the flow cytometer companies

• Support to researchers/private companies for the culturing, sterile and non-sterile sorting and data analyses for punctual needs

• Training of researchers for the culturing, sterile and non-sterile sorting and data analyses for recurrent needs.

• Project Preparation: assistance with labelling (dissociation, fixation, blocking, permeabilization, dilution of antibodies…), discussions with the researchers/customers about their model, their research question, and controls, assistance in the theoretical design of complex multicolor panels (tandems, brightness, co-expression…).

• Analysis of samples with multiple labelling (gating strategy…).

• Technology monitoring (software, fluorochromes, sample preparation).

• You will be encouraged to seek funding from public agencies or private partners and provide flow cytometry-based solutions to external clients.

• Pipelines setup, guide design, collaboration with the user.

• You will be asked to develop a genome-edition core facility for mammalian cells by establishing adapted CrispR-Cas protocols combined with cell sorting in the frame of research projects conducted at UNamur and possibly in private companies.

Skills/Qualifications1) DiplomasYou hold:

• an engineer degree or a master degree in science or another relevant discipline,

• AND a doctoral thesis during which you developed strong expertise in flow cytometry and ideally in genome editing.

• N.B. : Candidates who have not yet obtained the academic degree of Doctor can apply only if they are about to defend their doctoral thesis (at the latest within 3 months following the appointment).

2) Professional experienceAt least 2-year professional experience in flow cytometry.3) Required SkillsYou have the following skills:

• Excellent theoretical and practical knowledge of flow cytometery (analyzer and sorters), with expertise in designing complex flow cytometry-based experiments. The expertise of the candidate should be demonstrated by publications in peer-reviewed journals.

• Mastery of labelling techniques for (1) studying eukaryotic and bacterial cellular processes such as the cell cycle (G1, S, G2-M), ploidy, cell viability, and apoptosis, and (2) performing phenotyping (immune cells, organoids, intracellular signaling, mitochondrial potential…).

• Good experience with FlowJo software is a plus (training users on FlowJo).

• Multitasking abilities, excellent sense of priorities and organization, autonomy. You will have to manage several projects simultaneously: support in flow cytometry (analysis and cell sorting) for several facility users/projects.

• Strong team spirit: you will work with other team members.

• Strong service spirit: you must appreciate to collaborate on the projects of other researchers.

• Excellent communication abilities: you must be proficient in English (oral and written).

Knowledge and skills in bold will be assessed during the recruitment process.4) Advantages

• Good practice of cell biology tools (cell culture, immunofluorescence…).

• Good practice of biochemistry/molecular biology tools (cloning, DNA and protein electrophoresis, Western blotting…).

• Practical knowledge/experience of CRISPR technology using transfection/transduction. The experience of the candidate should be demonstrated by publications in peer-reviewed journals.

Contract: one-year fixed-term contract (100%)Starting date: as soon as possibleHow to applyThe application file, consisting of a detailed curriculum vitae, a cover letter and the completed application form, must be sent for April 26st at the latest. For more details, see Application URL.RequirementsAdditional InformationWork Location(s)Number of offers available 1 Company/Institute UNamur (Université de Namur) Country Belgium GeofieldWhere to apply WebsiteContact WebsiteSTATUS: EXPIRED

Expected salary

Location

Belgique

Job date

Mon, 04 Mar 2024 04:44:33 GMT

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