Job title:
Postdoctoral position (M/F): Peptide Targeting of siRNAs to RISC to Enhance Their Biological Activity
Company:
Job description
Offer DescriptionFive siRNAs are clinically approved, including 4 conjugated with saccharides. However, their therapeutic efficacy is limited by the very low proportion of siRNA that reaches the RNA Induced Silencing Complex (RISC) after internalization. The proposed work consists of intracellular targeting of siRNA to RISC. Increased loading of siRNAs into RISC will result in drastically increased biological activity at an equivalent dose. The proposed work involves evaluating new peptide-siRNA conjugates to target RISC. The evaluation of these conjugates includes the synthesis and purification of peptides, conjugation of peptides to siRNA and, characterization of the conjugates. Different linkers will be compared both in terms of interaction with recombinant proteins in vitro and silencing in cellular modelsPeptide purification
Conjugation of peptides to siRNA
Characterization of conjugates
Molecular interaction studies using Biolayer interferometry, FIDA, filter binding assay
Cell electroporation and silencing evaluationThe CBM (UPR 4301) is a CNRS research unit of 140 people where several groups work in synergy at the interface between physics, biology and chemistry ( ). The recruited person will carry out their activities within two teams. The “Macromolecular Assemblies and Complex Systems” team ( ) and the Synthetic Proteins and Bio-orthogonal Chemistry team ( ).
This recruitment is financed as part of the EMergence@CNRS Chimie 2025 project “Peptide targeting from siRNA to RISC”.Where to apply WebsiteRequirementsResearch Field Chemistry Education Level PhD or equivalentResearch Field Physics Education Level PhD or equivalentResearch Field Biological sciences Education Level PhD or equivalentLanguages FRENCH Level BasicResearch Field Chemistry » Biochemistry Years of Research Experience 1 – 4Research Field Physics » Biophysics Years of Research Experience 1 – 4Research Field Biological sciences » Biological engineering Years of Research Experience 1 – 4Additional InformationEligibility criteriaThe candidate must have skills in conjugation chemistry and analytical chemistry (LC-MS).
Experience in conjugation chemistry
Protein purification skills
Experience with nucleic acids would be a plus but is not a prerequisite
Ability to work autonomously and rigorouslyWebsite for additional job detailsWork Location(s)Number of offers available 1 Company/Institute Centre de Biophysique Moléculaire Country France City ORLEANS GeofieldContact CityORLEANS WebsiteSTATUS: EXPIREDShare this page
Expected salary
Location
Orléans, Loiret
Job date
Thu, 26 Dec 2024 02:37:19 GMT
To help us track our recruitment effort, please indicate in your email/cover letter where (vacanciesineu.com) you saw this job posting.
