Postdoc researcher in biology F/H

22 Sep 2023
Job Information

Organisation/Company

UNIVERSITE D’ORLEANS

Department

45

Research Field

Biological sciences » Biology

Researcher Profile

First Stage Researcher (R1)
Recognised Researcher (R2)

Country

France

Application Deadline

15 Oct 2023 – 12:00 (Europe/Paris)

Type of Contract

Temporary

Job Status

Full-time

Hours Per Week

37h30

Is the job funded through the EU Research Framework Programme?

Not funded by an EU programme

Is the Job related to staff position within a Research Infrastructure?

No

Offer Description

Context of the work:

In the early 21st century, it was demonstrated that plasma (physical plasma and not blood plasma) can be used for biomedical applications. Non equilibrium plasma has the ability to generate large population densities of reactive species, while keeping the overall gas temperature near room temperature (below 40°C), allowing the plasma to be in direct contact with living tissues with no risk of burning (cf the picture below). This singular feature led to the development of a new area, called Plasma Medicine. Research is now performed on many different topics including work on cancer treatment, wound healing, blood coagulation, dentistry, cosmetology, sterilization and decontamination, among others. The successes of plasmas have come so far from their versatility and their capacity to generate large amounts of reactive species combined with electric field, photons (IR, visible and UV) and charged particles. All these components can act on tissues and their combination induces synergetic effects (1)

 

A great number of the species produced by plasma are reactive oxygen and nitrogen species (RONS), such as atomic oxygen, ozone (O3 ), nitric oxide (NO) and hydroxide (OH), produced by the interaction of the plasma with air. These RONS are known to have various beneficial effects such as antibacterial and vasodilator properties. But surprisingly, the role of CO has so far been neglected in biological plasma treatment (2), while this molecule has many beneficial effects for human health and has a broad spectrum of biological activities such as anti-inflammatory, vasodilator, anti-apoptotic, and anti-proliferative effects, and can be easily produced by plasma by the dissociation of CO2 (3).

 

The work will be dedicated to the study of the effects of CO produced by plasma on wound healing. It is summarized into two tasks:

1)      In vitro study: determine safe conditions for cells involved in skin wound healing (viability/cytotoxicity assessment), measure the amount of CO accumulated in cells, evaluate the cell migration of cells with a scratch assay and evaluation of phagocytosis of macrophage cells.  

2)     In vivo study: study of wound heling on Black C57BL/6 mice, treatment tolerance (temperature of the skin, the weight and the heart rate of the mouse, histopathological analysis), histology will be conducted to monitor tissue morphology and to make sure that no adverse effect has been induced. Sections of wounds will be either stored for analysis of gene and protein expression of inflammatory markers (such as for instance IL-10 and IL-6 protein) or stained with antibodies to determine angiogenesis (formation of new blood vessels) and inflammation (such as VEGF protein and TGF beta).

 

This work will be a collaboration between the GREMI laboratory in Orleans and the INSERM laboratory in Creteil (University Paris-East). GREMI is a laboratory specialized on the study of plasma reactors for biomedical applications and is composed of plasma physicists and chemists. The team number 12 in Creteil is specialized in CO biology and is composed of biologists.

 

(1)      E. Carbone and C. Douat, Plasma Med., vol. 8, no. 1, pp. 93–120, 2018.

(2)      C. Douat, P. Escot Bocanegra, S. Dozias, É. Robert, and R. Motterlini, Plasma Process. Polym., vol. 18, no. 9, p. 2100069, Sep. 2021.

(3)      R. Motterlini and L. E. Otterbein, Nat. Rev. Drug Discov., vol. 9, no. 9, pp. 728–743, Sep. 2010.

 

Requirements

Research Field

Biological sciences » Biology

Education Level

PhD or equivalent

Skills/Qualifications

Mandatory : PhD in biology

Mandatory : Molecular biology : Western Blotting, Real Time PCR, ELISA, DNA isolation and DNA purification de DNA, RNA, proteins

Mandatory : Cellular models: cell growth of mouse and human endothelial, macrophages, fibroblasts and keratinocytes cells, fluorescent Microscopy, flow cytometry

Recommended : Animal disease model (wound healing, diabetic)

Recommended : Vascular biology, dermatology and measure of inflammation and immune markers (ELISA test, spectrophotometry)

Optional : Use of plasma reactor in plasma medicine field

Research Field

Biological sciences » Biology

Additional Information
Additional comments

Submit before 13/10/2022, a motivation letter, a resume, recommendation letters (optional) and the PhD defense certificate

 

Contact  : Claire Douat (GREMI), [email protected]

    Roberto Motterlini (INSERM), [email protected]

Work Location(s)

Number of offers available

1

Company/Institute

GREMI laboratory / INSERM

Country

France

City

Orléans / Créteil

Postal Code

45100

Geofield

Where to apply

Website

https://jobaffinity.fr/apply/4z4n1ca59y4qgzq87v

Contact

City

Orléans

Website

http://www.univ-orleans.fr/

Street

Château de la Source, Avenue du Parc Floral

Postal Code

45067

STATUS: EXPIRED