Postdoc CRISPR technologies in neurogenomics

Job title:

Postdoc CRISPR technologies in neurogenomics

Company:

Job description

Offer DescriptionYou will lead your own project to discover and characterize modifiers of disease pathways in iPSC models of amyotrophic lateral sclerosis (ALS), a fatal neurodegenerative disease that effects 1 in 350 people. Your work will leverage markers of ALS pathology that have already been established using a combination of human genetics analyses, omics / neuropathological profiling of postmortem patient tissue and iPSC studies.For the first phase of your project, you will implement a protocol to conduct FACS sorting of iPSC derived motor neurons based on key disease markers. You will then combine this protocol with a pooled genomewide CRISPR deletion screening platform that has already been optimized for mammalian cell culture systems. Through this, you will prioritize candidate modifiers of key ALS phenotypes. This prioritization process will also be informed by various technical validations, mechanistic hypotheses and by integrating your results with parallel genetic analyses of whole genome sequencing data from large numbers of ALS patients (in partnership with project collaborators).In the second phase of your project, you will dissect the mechanistic links between prioritized modifiers and ALS phenotypes. Through this you aim to provide new insights into disease mechanisms and establish novel targets for ALS translational research. The tools available to you to perform this work include a range of established ALS iPSC lines, isogenic controls, iPSC neuron/organoid culture facilities, microfluidic systems, cellular imaging (calcium, confocal, lightsheet) as well as standard biochemistry and neurobiology techniques. A range of assays for specific ALS phenotypes have also already been established. Your work will also be supported by interactions with colleagues that have experience in ALS, iPSC models, bioinformatics, genomics and a diverse areas of neuroscience.

Where to apply WebsiteRequirementsSpecific Requirements

• You are an established postdoctoral researcher or recently graduated PhD in a relevant scientific discipline and demonstrable experience in molecular cloning and iPSC models.
• Strong preference is given to candidates with a neuroscience background.
• You are motivated to grow your career by taking ownership of a research project focused on molecular mechanisms in neurodegenerative disease.
• Prior experience in cell sorting, in situ hybridization assays, lentiviral transduction, CRISPR technologies or bioinformatics is valued but not required.
• You are interested to combine iPSC models of ALS with high throughput perturbation assays and subsequent functional studies.
• You are a team player, you enjoy collaboration across disciplines and you are a good communicator.

Additional InformationBenefitsThe maximum salary for this position (36 – 36 hours) is € 5.504,00 gross per month based on full-time employment.In addition, we offer an annual benefit of 8.3%, holiday allowance, travel expenses and career opportunities. The are in accordance with the Cao University Medical Centers (UMC).Additional commentsContact our colleague:
Marie Hagen
088 75 688 05
Website for additional job detailsWork Location(s)Number of offers available 1 Company/Institute UMC Utrecht Country Netherlands City Utrecht Postal Code 3584CX Street Heidelberglaan 100 GeofieldContact CityUtrecht WebsiteStreetHeidelberglaan 100 Postal Code3584 CXSTATUS: EXPIREDShare this page

Expected salary

€5504 per month

Location

Utrecht

Job date

Fri, 01 Nov 2024 02:40:57 GMT

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