Explore the structural determinants of the autoimmunity against complement C1q in lupus patients H/F

Job title:

Explore the structural determinants of the autoimmunity against complement C1q in lupus patients H/F

Company:

Commissariat à l’Énergie Atomique

Job description

Entité de rattachement Le CEA est un acteur majeur de la recherche, au service des citoyens, de l’économie et de l’Etat.Il apporte des solutions concrètes à leurs besoins dans quatre domaines principaux : transition énergétique, transition numérique, technologies pour la médecine du futur, défense et sécurité sur un socle de recherche fondamentale. Le CEA s’engage depuis plus de 75 ans au service de la souveraineté scientifique, technologique et industrielle de la France et de l’Europe pour un présent et un avenir mieux maîtrisés et plus sûrs.Implanté au cœur des territoires équipés de très grandes infrastructures de recherche, le CEA dispose d’un large éventail de partenaires académiques et industriels en France, en Europe et à l’international.Les 20 000 collaboratrices et collaborateurs du CEA partagent trois valeurs fondamentales :

• La conscience des responsabilités
• La coopération
• La curiosité

Référence 2024-32737Description de l’unitéThe IBS is an internationally recognized research center in the field of integrated structural biology. Our institute explores the structure and dynamics of proteins and other biological macromolecules to understand how they function at the atomic level and in a cellular environment.
Biological phenomena – such as host cell defenses against infection, membrane transport, molecular mechanisms of cancer, adaptation of molecules to extreme environments, DNA repair, epigenetic regulation and programmed cell death – are highly complex. To understand these and other fundamental cellular processes, we aim to obtain an accurate description of the biological molecules involved by studying their structure, dynamics and interactions with molecular partners and by elucidating the relationship between their structure and function.
Current challenges in structural biology include the structure determination of large macromolecular assemblies and the understanding of protein dynamics within such assemblies or in a cellular environment. Such challenges require a multidisciplinary approach at the interface of biology, physics and chemistry, as well as powerful instruments and methods of analysis spanning different resolution scales, available at the institute or nearby. Establishing multidisciplinary collaborative networks is also critical for the success of our research.Description du posteDomaineBiologie, biophysique et biochimieContratPost-doctoratIntitulé de l’offreExplore the structural determinants of the autoimmunity against complement C1q in lupus patients H/FSujet de stageExplore the structural determinants of the autoimmunity against complement C1q in lupus patientsDurée du contrat (en mois)

18Description de l’offreOur team is dedicated to advancing the understanding of the complement system and its structural components, with a primary focus on autoimmune and chronic inflammatory diseases. Our current projects explore:– The interplay between alarmins and the classical complement pathway.– The novel roles of C1 proteases in the development of renal cancer.Our approach is multifaceted, combining structural analysis and interaction studies with the recombinant production of target proteins, including their fragments and relevant variants, using mammalian expression systems.Join our research team and embark on a cutting-edge project at the forefront of complement system biology. We are seeking a motivated and talented postdoctoral researcher to explore the antigenic determinants and the pathogenicity of the anti-C1q autoantibodies in a systemic lupus erythematosus – a paradigm of disease of systemic autoimmunity.You will have the following key responsibilities:– Recombinant Expression and Purification of C1q and its fragments: Initiate the project by producing and purifying complement C1q and its variants using advanced recombinant techniques.– ELISA Analysis: Utilize ELISAs to precisely identify the domains of C1q targeted by autoantibodies in sera from SLE patients and control groups.– Autoantibody Characterization: Conduct in-depth analysis of purified autoantibodies from SLE patient plasma to understand their interactions and implications in the disease pathophysiology.– Structural Exploration: Employ complementary structural approaches to explore how C1q conformation modulates epitope access and gain comprehensive insights into the behavior and function of C1q and their perturbation by autoantibody interactions.Profil du candidat– Ph.D. in Biochemistry, Molecular Biology, or a related field.– Strong experience in recombinant protein expression and purification.– Proficiency in ELISA and other immunoassays.– Familiarity with structural biology techniques will be greatly appreciated.– Passion for scientific discovery and a collaborative spirit.In accordance with the commitments made by the CEA in favor of the integration of people with disabilities, this job is open to everyone. The CEA offers accommodations and/or organizational possibilities for the inclusion of disabled workersLocalisation du posteSiteGrenobleLocalisation du posteFrance, Auvergne-Rhône-Alpes, Isère (38)VilleGRENOBLECritères candidatLanguesAnglais (Courant)DemandeurDisponibilité du poste01/09/2024

Expected salary

Location

Isère

Job date

Wed, 21 Aug 2024 02:21:25 GMT

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