ASSISTANT PROFESSOR/ Post doc position

Job title:

ASSISTANT PROFESSOR/ Post doc position

Company:

Job description

Offer DescriptionWe are looking for a qualified postdoctoral researcher to join our dynamic research team to conduct advanced research in metabolic engineering of S.cerevisiae (backer’s yeast) The person employed in this position will be responsible for the implementation of the research project and writing scientific publications.The project will be focused on obtaining genetically modified strains of S.cerevisiae and characterising them. Genetic manipulations will require the candidate to design and prepare constructs for the CRISPR/Cas genome editing of S.cerevisiae. Genetic manipulations are aimed at obtaining a strain capable of simultaneous biosynthesis of mixed PHB/PLA (poly((R-3 hydroxybutyrate/polylactide) polymers. As part of the research conducted so far, the project leader’s team has developed a strain with a favourable metabolic profile, which will serve as the starting point for genetic manipulation and for the production of the polymers. The effects of genome editing will be verified at the level of transcription using real-time PCR, at the level of metabolism by determining concentration of intracellular metabolites, and in the culture media, as well as at the level of activity of selected enzymes, in the synthetic pathways as well as their cellular levels using the western blot immunodetection method. For the most promising strains, quantitative tests of the synthesis of polylactide and hydroxybutyrate will be carried out, as well as the optimisation of polymer production efficiency using a laboratory bioreactor. The extraction and characterisation of polymers in terms of their composition will be carried out using chromatographic methods and MALDI-TOF mass spectrometry/ NMR. Optimising the polymer extraction process from S.cerevisiae will be a key step in the project, followed by an evaluation of the strategy in designing a synthetic hybrid microorganism as an efficient polymer producer. The project will be caried out over several interactions to obtain the optimal solution.The postdoctoral researcher will be working in a friendly environment at the Chair of Drug and Cosmetics Biotechnology at the Faculty of Chemistry WUT.RequirementsResearch Field Biological sciences » Biological engineering Education Level PhD or equivalentResearch Field Biological sciences » Biology Education Level PhD or equivalentResearch Field Chemistry » Biochemistry Education Level PhD or equivalentResearch Field Technology » Biotechnology Education Level PhD or equivalentSkills/Qualifications1. PhD degree in a field related to the announced project (preferred disciplines are: biological sciences, life sciences, bioscience, biological engineering).2. documented experience in conducting research in the area which may be confirmed by scientific publications or references.3. The candidate is expected to have an experience in at least two of the research directions listed below:– microbial genome editing using the CRISPR/Cas technique– molecular biology involving microorganisms (bacteria, yeast cells), working with DNA/RNA– experience in using PCR/RT-PCR techniques– experience of enzymatic assays– analysis of PHB and PLA polymers– extraction of polymers from microorganisms and fungi, and the chromatographic analysis of polymers4. excellent communication skills, both written and oral, in English.5. ability to cooperate effectively with other members of the research team and openness to sharing knowledge and experience.6. commitment to conducting innovative research and willingness to develop in a given scientific field.Additional advantage:– Experience in writing scientific publicationLanguages ENGLISH Level GoodLanguages POLISH Level GoodResearch Field Biological sciences » Biological engineeringBiological sciences » BiologyChemistry » BiochemistryTechnology » Biotechnology Years of Research Experience 4 – 10Additional InformationAdditional commentsMore information is available from :dr hab. inż. Malgorzata Adamczyk, prof. uczelni, Gmach Technologii Chemicznej, ul. Koszykowa 75,00-662 Warszawa, pok. 103A, tel.: (+48 22) 234-7677,e-mail:Work Location(s)Number of offers available 1 Company/Institute Warsaw University of Technology, Faculty of Chemistry Chair of Drug and Cosmetics Biotechnology Country Poland City Warsaw Postal Code 00 – 664 Street Noakowskiego 3 GeofieldWhere to apply E-mail[email protected]Contact CityWarsaw WebsiteStreetNoakowskiego 3 Postal Code00 – 664 E-Mail[email protected]STATUS: EXPIRED

Expected salary

Location

Warszawa, mazowieckie

Job date

Fri, 19 Apr 2024 03:34:54 GMT

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