Job title:
Doctorat (M/F) – Synthetic protein chemistry applied to TIP60 coregulator of gene transcription
Company:
Job description
Offer DescriptionThe project will be conducted in the Biosystems chemistry group within the interdisciplinary unit UMR 7242 Biotechnology and Cellular Signalling directed by CNRS and the University of Strasbourg and located at the Ecole Superieure de Biotechnologie de Strasbourg (ESBS) at the Illkirch campus. The research unit is composed of 8 research groups working on different topics such as integrity of the genome and membrane signalling. The research approaches combine functional genomics and chemical biology. The research in our team focuses on the application of chemical protein synthesis. The necessary facilities for conducting the work will be provided such as chemical synthesis of peptides by solid-phase synthesis, recombinant protein expression, characterization by HPLC and mass-spectrometry, as well as state-of-the-art NMR spectroscopy. Currently, our team is composed of 1 Professor, 1 Assistant Professor, 1 postdoctoral researcher and 4 PhD students.
We are searching for a highly motivated candidate, skilled in organic synthesis and eager to practice biological-related experiments. Previous experience in peptide synthesis will be a strong advantage. The candidate will integrate into the team of Biosystelms Chemistry at UMR 7242 and will be enrolled in the ED222 doctoral school in Chemistry, which requires to follow the rules of the doctoral education.
For more information, please, see web-site: .The doctoral thesis work being part of the ANR project (C-TIP60 – Structure and function of the human TIP60/p400 co-activator) will be supervised by Prof. Vladimir Torbeev and carried out at the CNRS research unit UMR7242 Biotechnology and Cellular Signalling. Three other teams will be taking part in the project: A. Ben Shem (P. Schultz’s team at IGBMC, coordinator), A. Hamiche (IGBMC) in Strasbourg and J.-P. Concordet (U1154/UMR7196 Museum National d’Histoire Naturelle) in Paris, possessing expertise in biochemistry, structural biology, epigenetics and cellular engineering. The doctoral student will be responsible for the semi-synthesis of histones and activation domains of transcriptional coactivators possessing mimics of post-translational modifications, reacting functionalities and conformationally modified amino acids, following by reconstitution of nucleosomes into transcription-active complexes. Protein semi-synthesis is a methodology for protein engineering that combines recombinant protein expression and chemical synthesis of polypeptides. The recombinant and chemically synthesized protein fragments will be conjugated by native chemical ligation as well as enzymatically-catalyzed ligations. Cryo-EM will be used to solve their structures and relevant biological functions will be studied.
Publications related to the project:
Bauer V, Schmidtgall B, Gógl G, et al. Conformational editing of intrinsically disordered protein by α-methylation. Chem Sci. 2021;12(3):1080-1089. doi:10.1039/d0sc04482b
Fréchard A, Faux C, Hexnerova R, et al. The structure of the NuA4-Tip60 complex reveals the mechanism and importance of long-range chromatin modification. Nat Struct Mol Biol. 2023;30(9):1337-1345. doi:10.1038/s41594-023-01056-xWhere to apply WebsiteRequirementsResearch Field Chemistry Education Level PhD or equivalentResearch Field Biological sciences Education Level PhD or equivalentResearch Field Pharmacological sciences Education Level PhD or equivalentLanguages FRENCH Level BasicResearch Field Chemistry Years of Research Experience NoneResearch Field Biological sciences Years of Research Experience NoneResearch Field Pharmacological sciences Years of Research Experience NoneAdditional InformationAdditional commentsWe are looking for a candidate with a Master’s degree in chemistry or biochemistry, with in particular: solid knowledge in organic chemistry, motivated by interdisciplinary experimental work, curiosity for research, critical thinking, good writing and communication skills. Candidates must provide CV, cover letter and the email contact of the referees. Academic transcripts will be requested. Website for additional job detailsWork Location(s)Number of offers available 1 Company/Institute Biotechnologie et signalisation cellulaire Country France City ILLKIRCH GRAFFENSTADEN GeofieldContact CityILLKIRCH GRAFFENSTADEN WebsiteSTATUS: EXPIREDShare this page
Expected salary
Location
Illkirch-Graffenstaden, Bas-Rhin
Job date
Sat, 10 Aug 2024 06:14:26 GMT
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